Meiji Pharmaceutical University 4 articles published in JoVE Genetics Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment Katsunori Yanai1, Shohei Kaneko1, Hiroki Ishii1, Akinori Aomatsu1,2, Kenichi Ishibashi3, Yoshiyuki Morishita1 1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University We present a method for evaluating microRNA expression in the kidney and serum of mice with age-dependent renal impairment by quantitative reverse-transcription polymerase chain reaction. Genetics Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction Katsunori Yanai1, Shohei Kaneko1, Hiroki Ishii1, Akinori Aomatsu1,2, Kiyonori Ito1, Keiji Hirai1, Susumu Ookawara1, Kenichi Ishibashi3, Yoshiyuki Morishita1 1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University We describe a method for evaluating the microRNA expression in the kidneys of mice with unilateral ureteral obstruction (UUO) by quantitative reverse-transcription polymerase chain reaction. This protocol is suitable for studying kidney microRNA expression profiles in mice with UUO and in the context of other pathological conditions. Immunology and Infection Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice Shohei Kaneko1, Katsunori Yanai1, Hiroki Ishii1, Akinori Aomatsu1,2, Kiyonori Ito1, Keiji Hirai1, Susumu Ookawara1, Kenichi Ishibashi3, Yoshiyuki Morishita1 1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University microRNAs are involved in the pathogenesis of IgA nephropathy. We have developed a reliable method for detecting microRNA expression levels in the kidneys of an IgA nephropathy mouse model (HIGA mice). This new method will facilitate to check for miRNAs involvement in IgA nephropathy. Genetics Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR Keiji Hirai1, Hiromichi Yoshizawa2, Toshimi Imai2, Yusuke Igarashi2, Ichiro Hirahara2, Susumu Ookawara2, Kenichi Ishibashi3, Yoshiyuki Morishita1 1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Nephrology, Department of Internal Medicine, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University Here we present a protocol for the detection of microRNA expression in rat peritoneal membrane using quantitative real-time reverse-transcription polymerase chain reaction. This method is suitable for studying the microRNA expression profile in rat peritoneal membrane in several pathological conditions.
