3 articles published in JoVE
Novel Methods for Intranasal Administration Under Inhalation Anesthesia to Evaluate Nose-to-Brain Drug Delivery Takanori Kanazawa*1, Mitsuyoshi Fukuda*1, Naoto Suzuki1, Toyofumi Suzuki1 1Laboratory of Pharmaceutics, School of Pharmacy, Nihon University Here, we describe two novel methods of stable intranasal administration under inhalation anesthesia with minimal physical stress for experimental animals. We also describe a method for quantitative evaluation of drug distribution levels in the brain via the nose-to-brain pathway using radiolabeled [14C]-inulin as a model substrate of water-soluble macromolecules.
Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies M. Rezaul Karim1, Tomohiro Haruta2, Taro Matsumoto3, Hiroki Oda2, Hiroaki Taniguchi3,4,5 1Biotechnology and Genetic Engineering Department, Jahangirnagar University, 2JT Biohistory Research Hall, 3Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, 4Graduate School of Life and Medical Sciences, Doshisha University, 5Institute of Genetics and Animal Breeding of the Polish Academy of Sciences Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.
An Efficient Method to Obtain Dedifferentiated Fat Cells Hiroaki Taniguchi*1, Tomohiko Kazama*1, Kazuhiro Hagikura*1, Chii Yamamoto1, Minako Kazama1, Yuki Nagaoka1, Taro Matsumoto1 1Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University We have modified the conditions for DFAT cell generation and provide herein information regarding the use of an improved growth medium for the production of these cells.