2 articles published in JoVE
Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants Sota Iwatani1, Makiko Yoshida2, Keiji Yamana1, Daisuke Kurokawa1, Jumpei Kuroda1, Khin Kyae Mon Thwin1, Suguru Uemura1, Satoru Takafuji1, Nanako Nino1, Tsubasa Koda, Masami Mizobuchi4, Masahiro Nishiyama1, Kazumichi Fujioka1, Hiroaki Nagase1, Ichiro Morioka5, Kazumoto Iijima1, Noriyuki Nishimura1 1Department of Pediatrics, Kobe University Graduate School of Medicine, 2 Human umbilical cord (UC) can be obtained during the perinatal period as a result of preterm, term, and postterm delivery. In this protocol, we describe the isolation and characterization of UC-derived mesenchymal stem cells (UC-MSCs) from fetuses/infants at 19-40 weeks of gestation.
Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA Masaki Miya1, Toshifumi Minamoto2, Hiroki Yamanaka3, Shin-ichiro Oka4, Keiichi Sato4, Satoshi Yamamoto2, Tetsuya Sado1, Hideyuki Doi5 1Department of Ecology and Environmental Sciences, Natural History Museum and Institute, Chiba, 2Graduate School of Human Development and Environment, Kobe University, 3Faculty of Science and Technology, Ryukoku University, 4Okinawa Churashima Research Center, 5Graduate School of Simulation Studies, University of Hyogo We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.