RIKEN Cluster for Pioneering Research 1 article published in JoVE Biology TChIP-Seq: Cell-Type-Specific Epigenome Profiling Mari Mito1,2, Mitsutaka Kadota3, Shinichi Nakagawa1,4, Shintaro Iwasaki2,5 1RNA Biology Laboratory, RIKEN, 2RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research, 3Laboratory for Phyloinformatics, RIKEN Center for Biosystems Dynamics Research, 4RNA Biology Laboratory, Faculty of Pharmaceutical Sciences, Hokkaido University, 5Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo We describe a step-by-step protocol for tandem chromatin immunoprecipitation sequencing (tChIP-Seq) that enables the analysis of cell-type-specific genome-wide histone modification.
