Osaka Prefecture University 1 article published in JoVE Bioengineering Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations Masaya Hagiwara1, Rina Nobata2, Tomohiro Kawahara3 1NanoSquare Research Institute, Osaka Prefecture University, 2Department of Bioscience and Informatics, Osaka Prefecture University, 3Department of Biological Functions Engineering, Kyushu Institute of Technology We present a procedure for controlling the initial cell cluster shape in a 3D extracellular matrix to obtain a repeatable pattern formation. A cubic device containing two different hydrogels is employed to achieve multi-directional imaging for tissue pattern formation.
