2 articles published in JoVE
Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins. Razan E. Ahmed*1, Nawin Chanthra*1, Tatsuya Anzai1,2, Keiichiro Koiwai3,4, Tomoki Murakami4, Hiroaki Suzuki4, Yutaka Hanazono1, Hideki Uosaki1 1Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 2Department of Pediatrics, Jichi Medical University, 3Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 4Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University This method can be used to examine sarcomere shortening using pluripotent stem cell-derived cardiomyocytes with fluorescent-tagged sarcomere proteins.
Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O Megumi Kuroiwa1, Keitaro Fukushima2,3, Kazuma Hashimoto2, Yukiko Senga4, Tsubasa Sato4, Chie Katsuyama5, Yuichi Suwa1 1Department of Applied Chemistry, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 1Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, 2Faculty & Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, 3Center for Ecological Research, Kyoto University, 4Department of Chemistry, Faculty of Science, Toho University, 5Graduate School of Integrated Arts and Sciences, Hiroshima University A series of methods to determine the potential DNRA rate based on 14NH4+/15NH4+ analyses is provided in detail. NH4+ is converted into N2O via several steps and analyzed using quadrupole gas chromatography–mass spectrometry.