Shonai Regional Industry Promotion Center 1 article published in JoVE Cancer Research Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry Ami Maruyama1,2, Kenjiro Kami3, Kazunori Sasaki3, Hajime Sato3, Yuzo Sato1,2, Katsuya Tsuchihara4, Hideki Makinoshima1,2,4 1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory, National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center The purpose of this article is to describe a protocol for extraction of aqueous metabolites from cultured adherent cells for metabolomic analysis, particularly, capillary electrophoresis-mass spectrometry.
