Laboratory for Oral Health Science 1 article published in JoVE Immunology and Infection Purification of a High Molecular Mass Protein in Streptococcus mutans Takatoshi Murata1, Mamiko Yamashita1, Masao Ishikawa1,2, Koji Shibuya2, Nobuhiro Hanada1 1Department of Translational Research, Tsurumi University School of Dental Medicine, 2Laboratory for Oral Health Science Described here is a simple method for the purification of a gene product in Streptococcus mutans. This technique may be advantageous in the purification of proteins, especially membrane proteins and high molecular mass proteins, and can be used with various other bacterial species.