3 articles published in JoVE
Electroporation-mediated RNA Interference Method in Odonata Genta Okude1,2, Takema Fukatsu1,2,3, Ryo Futahashi2 1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 2Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 3Graduate School of Life and Environmental Sciences, University of Tsukuba We provide a detailed protocol for electroporation-mediated RNA interference in insects of the order Odonata (dragonflies and damselflies) using the blue-tailed damselfly (Ischnura senegalensis: Coenagironidae: Zygoptera) and the pied skimmer dragonfly (Pseudothemis zonata: Libellulidae: Anisoptera).
Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System Kazuma Sekiba1,2, Motoyuki Otsuka1, Kazuhiko Koike1 1Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, 2Research Fellow of Japan Society for the Promotion of Science Here, we present a method for screening anti-hepatitis B viral agents that inhibit the HBx-DDB1 interaction using a split luciferase assay system. This system allows easy detection of protein-protein interactions and is suitable for identifying inhibitors of such interactions.
Technical Aspects of the Mouse Aortocaval Fistula Kota Yamamoto1,2, Xin Li1,3, Chang Shu3, Tetsuro Miyata2, Alan Dardik1,4 1The Department of Surgery and the Interdepartmental Program in Vascular Biology and Therapeutics, Yale University, 2Department of Vascular Surgery, The University of Tokyo, 3Department of Vascular Surgery, Central South University, 4VA Connecticut Healthcare Systems The goal is to produce an arteriovenous fistula that is simple and reproducible. This method does not use sutures or glue adhesive. Therefore the samples can be used with the least amount of foreign materials for analysis.