Shiga University of Medical Science 1 article published in JoVE Biology An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice Mariko Omatsu-Kanbe1, Ryo Fukunaga1, Xinya Mi1, Hiroshi Matsuura1 1Department of Physiology, Shiga University of Medical Science We developed a simple method for isolating high quality individual mouse heart cells by the antegrade perfusion technique. This method is Langendorff-free and useful for isolating ventricular and atrial myocytes or interstitial cells, such as cardiac fibroblasts or progenitors.
