Marine Works Japan Ltd. 1 article published in JoVE Biology Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy Masashi Yamaguchi1, Azusa Takahashi-Nakaguchi1, Katsuyuki Uematsu2, Masaki Taguchi2, Michiyo Sato-Okamoto1, Hiroji Chibana1 1Medical Mycology Research Center, Chiba University, 2Marine Works Japan Ltd. Here we show how to use the sandwich freezing device for rapid freezing of biological specimens, including bacteria, yeast, cultured cells, isolated cells, animal and human tissues, and viruses. We also show how to prepare specimens for ultrathin sectioning after rapid freezing.
