Chonbuk National University 5 articles published in JoVE Genetics Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay Ju-Chun Chang1, Se Jin Lee2, Jae Su Kim2, Chung-Hsiung Wang3, Yu-Shin Nai1 1Department of Biotechnology and Animal Science, National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences, Chonbuk National University, 3Department of Entomology, National Taiwan University This protocol describes a heat shock-induced protein expression system (pDHsp/V5-His/sf9 cell system), which can be used for either expressing foreign proteins or evaluating the anti-apoptotic activity of potential foreign proteins and their truncated amino acids in insect cells. Engineering Experimental Implementation of a New Composite Fabrication Method: Exposing Bare Fibers on the Composite Surface by the Soft Layer Method Dongyoung Lee1, Dai Gil Lee1, Jun Woo Lim2 1Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 2LANL-CBNU Engineering Institute Korea, Chonbuk National University A protocol to expose bare fibers on the composite surface by eliminating resin rich area is presented. The fibers are exposed during fabrication of the composites, not by the post surface treatment. The exposed carbon composites exhibit high electrical conductivity in the through-thickness direction and high mechanical property. Neuroscience Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila Sangyun Jeong1 1Department of Molecular Biology, Chonbuk National University This work details a standard immunohistochemistry method to visualize motor neuron projections of late stage-16 Drosophila melanogaster embryos. The filleted preparation of fixed embryos stained with FasII antibody provides a powerful tool to characterize the genes required for motor axon pathfinding and target recognition during neural development. Medicine An Anesthesia, Surgery, and Harvest Method for the Evaluation of Transpedicular Screws Using an In Vivo Porcine Lumbar Spine Model Young Jae Moon1, Jong-Kil Kim2, Hong-Geun Oh3, Ji-Hun Kang4, Gun-Joo Park1, Kwang-Bok Lee1 1Department of Orthopaedic Surgery, Chonbuk National University School of Medicine, Chonbuk National University Hospital, 2Department of Orthopaedic Surgery, University of Seonam College of Medicine, Presbyterian Medical Center, 3White Oaks West Animal Hospital, 4Department of Emergency Medicine, Inje University Busan Paik Hospital Here, we introduce a method to evaluate transpedicular screws by using an in vivo porcine lumbar spine model. Engineering Visible-light Induced Reduction of Graphene Oxide Using Plasmonic Nanoparticle Dinesh Kumar1, Ah-Reum Lee1, Sandeep Kaur2, Dong-Kwon Lim1 1KU-KIST Graduate School of Converging Science and Technology, Korea University, 2Department of BIN Fusion Technology, Chonbuk National University A simple protocol for the preparation of reduced graphene oxide using visible light and plasmonic nanoparticle is described.