Korea Research Institute of Bioscience and Biotechnology 6 articles published in JoVE Cancer Research Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells Jina Lee*1,2, Joo-Eun Lee*3, Seil Kim1,4,5, Dukjin Kang1, Hee Min Yoo1 1Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), 2College of Pharmacy, Chungnam National University (CNU), 3Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 4Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), 5Department of Bio-Analysis Science, University of Science & Technology (UST) Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways. Bioengineering Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock Dong Wei1,2, Jinjie Gu1, Zhongxi Zhang1, Chenhong Wang1, Dexin Wang1, Chul Ho Kim3, Biao Jiang1, Jiping Shi1,4, Jian Hao1 1Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, 2University of Chinese Academy of Sciences, 3Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 4School of Life Science and Technology, ShanghaiTech University Bamboo powder was pretreated with NaOH and enzymatically hydrolyzed. The hydrolysate of bamboo was used as the feedstock for 2,3-butanediol, R-acetoin, 2-ketogluconic acid, and xylonic acid production by Klebsiella pneumoniae. Genetics Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow Gajendra W. Suryawanshi*1,2, Song Xu*3, Yiming Xie1, Tom Chou3, Namshin Kim4, Irvin S. Y. Chen1,5, Sanggu Kim6 1UCLA AIDS Institute, University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics, University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics, University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups, Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine, University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University (OSU) This manuscript describes the experimental procedure and software analysis for a bidirectional integration site assay that can simultaneously analyze upstream and downstream vector-host junction DNA. Bidirectional PCR products can be used for any downstream sequencing platform. The resulting data are useful for a high-throughput, quantitative comparison of integrated DNA targets. Biochemistry Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer Haseong Kim*1, Gui Hwan Han*1, Yaoyao Fu1, Jongsik Gam2, Seung Goo Lee1,3 1Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 2College of Interdisciplinary & Creative Studies, Konyang University, 3Biosystems and Bioengineering Program, University of Science and Technology This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content. Genetics Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat Haeyoung Jeong*1, Sang J. Lee*2, Pil Kim3 1Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Bioengineering (KRIBB), 3Department of Biotechnology, The Catholic University of Korea Here, we present a protocol to obtain adaptive laboratory evolution of microorganisms under conditions using chemostat culture. Also, genomic analysis of the evolved strain is discussed. Biology Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System Haseong Kim1, Kil Koang Kwon1, Wonjae Seong1, Seung-Goo Lee1,2 1Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 2Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, South Korea This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).
