2 articles published in JoVE
Neurobehavioral Assessments in a Mouse Model of Neonatal Hypoxic-ischemic Brain Injury MinGi Kim1,2, Ji Hea Yu1, Jung Hwa Seo1,2, Yoon-Kyum Shin1,2, Soohyun Wi1,2, Ahreum Baek1,3, Suk-Young Song1,5, Sung-Rae Cho1,2,4,5 1Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, 2Brain Korea 21 PLUS Project for Medical Science, Yonsei University, 3Department of Rehabilitation Medicine, Yonsei University Wonju College of Medicine, 4Rehabilitation Institute of Neuromuscular Disease, Yonsei University College of Medicine, 5Graduate Program of NanoScience and Technology, Yonsei University We performed unilateral carotid artery occlusion on postnatal day 7-10 CD-1 mouse pups to create a neonatal hypoxic-ischemic (HI) model and investigated the effects of HI brain injury. We studied neurobehavioral functions in these mice compared to non-operated normal mice.
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer Alessandra Merenda1,2, Amanda Andersson-Rolf1,2, Roxana C. Mustata1, Taibo Li1, Hyunki Kim3, Bon-Kyoung Koo1,2 1Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge, 2Department of Genetics, University of Cambridge, 3Department of Pathology, Yonsei University College of Medicine This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this method.