3 articles published in JoVE
Effects of Exposure of Formaldehyde to a Rat Model of Atopic Dermatitis Induced by Neonatal Capsaicin Treatment Rafael Taeho Han1, Seung Keun Back2, JaeHee Lee1, Hye young Kim1, Hee Jin Kim3, Heung Sik Na1 1Neuroscience Research Institute & Department of Physiology, College of Medicine, Korea University, 2Department of Pharmaceutics and Biotechnology, College of Medical Engineering, Konyang University, 3Division of Biological Science and Technology, Science and Technology College, Yonsei University Wonju Campus We describe here methods for neonatal capsaicin treatment to induce atopic dermatitis in rats and exposure of vaporized formaldehyde to investigate the effects of formaldehyde inhalation on atopic dermatitis.
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer Haseong Kim*1, Gui Hwan Han*1, Yaoyao Fu1, Jongsik Gam2, Seung Goo Lee1,3 1Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 2College of Interdisciplinary & Creative Studies, Konyang University, 3Biosystems and Bioengineering Program, University of Science and Technology This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.
Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77 Pierre Leblanc1, Minho Moon1,2, Woori Kim1, Inhye Jeong1, Chun-Hyung Kim1, Kwang-Soo Kim1 1Molecular Neurobiology Laboratory, Psychiatry, McLean Hospital, 2Department of Biochemistry, College of Medicine, Konyang University Here, we selectively target antibodies against a specific member of a highly conserved family of proteins by immunizing animals with their most divergent regions followed by removing cross reactive antibodies by pre-adsorption.