Ulsan National Institute of Science and Technology (UNIST)

4 articles published in JoVE

• Biology

  
  Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis Chi-Ju Kim*^1,2, Morgan D. Kuczler*¹, Liang Dong^1,3, Junyoung Kim^2,4, Sarah R. Amend¹, Yoon-Kyoung Cho^2,4, Kenneth J. Pienta^{1 1}The Brady Urological Institute, Johns Hopkins University School of Medicine, ²Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), ³Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, ⁴Center for Soft and Living Matter, Institute for Basic Science (IBS) Extracellular vesicles (EVs) contribute to cellular biology and intercellular communications. There is a need for practical assays to visualize and quantify EVs uptake by the cells. The current protocol proposes the EV uptake assay by utilizing three-dimensional fluorescence imaging via confocal microscopy, following EV isolation by a nano-filtration-based microfluidic device.

• Chemistry

  
  Key Factors Affecting the Performance of Sb₂S₃-sensitized Solar Cells During an Sb₂S₃ Deposition via SbCl₃-thiourea Complex Solution-processing Yong Chan Choi¹, Sangl Il Seok², Eunjeong Hwang¹, Dae-Hwan Kim^{1 1}Convergence Research Center for Solar Energy, Daegu Gyeongbuk Institute of Science and Technology (DGIST), ²Perovtronics Research Center, School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST) This work provides a detailed experimental procedure for the deposition of Sb2S3 on a mesoporous TiO2 layer using a SbCl3-thiourea complex solution for applications in Sb2S3-sensitized solar cells. This article also determines key factors governing the deposition process.

• Environment

  
  Biofilm Removal Using Carbon Dioxide Aerosols without Nitrogen Purge Seongkyeol Hong¹, Jaesung Jang^{1 1}School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST) Biofilms on surfaces can be effectively and rapidly removed by using a periodic jet of carbon dioxide aerosols without a nitrogen purge.

• Bioengineering

  
  Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood Yang-Seok Park¹, Vijaya Sunkara¹, Yubin Kim¹, Won Seok Lee^1,2, Ja-Ryoung Han^1,3, Yoon-Kyoung Cho^{1,4 1}Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea, ²Agency for Defense Development (ADD), Daejeon, Republic of Korea, ³KOGAS (Korea Gas Corporation) Research Institute, ⁴Center for Soft and Living Matter, Institute for Basic Science (IBS) This protocol demonstrates how to achieve femto molar detection sensitivity of proteins in 10 µL of whole blood within 30 min. This can be achieved by using electrospun nanofibrous mats integrated in a lab-on-a-disc, which offers high surface area as well as effective mixing and washing for enhanced signal-to-noise ratio.