2 articles published in JoVE
ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging Eunsoo Lee1, Woong Sun1 1Department of Anatomy, Korea University College of Medicine ACT-PRESTO (active clarity technique–pressure related efficient and stable transfer of macromolecules into organs) enables rapid, efficient, but inexpensive tissue clearing and rapid antibody penetration into cleared tissues by passive diffusion or pressure-assisted delivery. Using this method, a wide range of tissues can be cleared, immunolabeled by multiple antibodies, and volume-imaged.
Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone Joo Yeon Kim1, Ju-Hyun Lee1, Woong Sun1 1Department of Anatomy, Korea University College of Medicine Establishing culture systems for the expansion of adult neural stem cells (aNSCs) allows for the examination and application of aNSCs for therapy. The subcallosal zone (SCZ) has recently been recognized as a novel neuroblast-forming region in adult mice. Here, methods for the isolation, expansion, and differentiation of SCZ-aNSCs are described.