3 articles published in JoVE
Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification Sura Kim1, Jayoung Ku1,2, Jaemin Park1, Raisa Kharbash1,2, Sheng Li1 1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 2KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST) A protocol for the preparation of poly(pentafluorophenyl acrylate) (poly(PFPA)) grafted silica beads is presented. The poly(PFPA) functionalized surface is then immobilized with antibodies and used successfully for the protein separation through immunoprecipitation.
Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning Jonghee Yoon1, YoungJu Jo2,3,4,7, Young Seo Kim3,4,5, Yeongjin Yu2,3, Jiyeon Park6, Sumin Lee4, Wei Sun Park2,3, YongKeun Park2,3,4 1Department of Physics, University of Cambridge, 2Department of Physics, Korea Advanced Institute of Science and Technology, 3KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology, 4Tomocube, Inc., 5Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 6Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 7Department of Applied Physics, Stanford University We describe a protocol for the label-free identification of lymphocyte subtypes using quantitative phase imaging and a machine learning algorithm. Measurements of 3D refractive index tomograms of lymphocytes present 3D morphological and biochemical information for individual cells, which is then analyzed with a machine-learning algorithm for identification of cell types.
Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture Jaejung Son1, Chae Yun Bae1, Je-Kyun Park1 1Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST) We describe the fabrication of micropatterned hydrogel sheets using a simple process, which can be assembled and manipulated in a freestanding form. Using these modular hydrogel sheets, a simple macro-scaled 3D cell culture system can be generated with a controlled cellular microenvironment.