2 articles published in JoVE
Assessing Neuroprotective Effects of Glycyrrhizae Radix et Rhizoma Extract Using a Transient Middle Cerebral Artery Occlusion Mouse Model Se-Eun Lee*1, Chiyeon Lim*2, Minji Lee1, Chang-Hyun Kim2, Hyungwoo Kim1, Byoungho Lee3, Suin Cho1 1School of Korean Medicine, Pusan National University, 2College of Medicine, Dongguk University, 3Kyunghee Naseul Korean Medicine Clinic In this study, we modify an existing experimental method to obtain more reproducible results, by establishing a middle cerebral artery occlusion (MCAO) mouse model. Oral administration of Glycyrrhizae Radix et Rhizome (GR) methanol extract (GRex), following stroke induction, significantly decreased total infarction volume relative to the untreated control group.
Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli Dong-Eog Kim1, Jeong-Yeon Kim1, Su-Kyoung Lee1, Ju Hee Ryu2, Ick Chan kwon2, Cheol-Hee Ahn3, Kwangmeyung Kim2, Dawid Schellingerhout4 1Molecular Imaging and Neurovascular Research Laboratory, Dongguk University College of Medicine, 2Biomedical Research Center, Korea Institute of Science and Technology, 3Research Institute of Advanced Materials, Department of Materials Science and Engineering, Seoul National University, 4Departments of Radiology and Cancer Systems Imaging, University of Texas M.D. Anderson Cancer Center This protocol describes the application of combined near-infrared fluorescent (NIRF) imaging and micro-computed tomography (microCT) for visualizing cerebral thromboemboli. This technique allows the quantification of thrombus burden and evolution. The NIRF imaging technique visualizes fluorescently labeled thrombus in excised brain, while the microCT technique visualizes thrombus inside living animals using gold-nanoparticles.