Pukyong National University 1 article published in JoVE Immunology and Infection Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation Jun-O Jin1,2, Soyeong Jang3, Hyehyun Kim4, Junghwan Oh4, Sungbo Shim5, Minseok Kwak3,4, Peter C.W. Lee6 1Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, 2Department of Medical Biotechnology, Yeungnam University, 3Department of Chemistry, Pukyong National University, 4Marine-integrated Bionics Research Center, Pukyong National University, 5Department of Biochemistry, Chungbuk National University, 6Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center Experimental procedures for the subsequent extraction of lymphatic tissues to test lymphoid dendritic cell activation are described after treatment of an immunostimulating nanomaterial.