Inje University 5 articles published in JoVE Engineering Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads Hong Jin Choi1, Byung Woo Kim2, SangWook Lee3, Ok Chan Jeong1,2,4 1Department of Digital Anti-Aging Health Care, Inje University, 2Micro device Lab Co., Ltd, 3Biohealth Products Research Center (BPRC), Inje University, 4Department of Biomedical Engineering, Inje University This paper describesthe fabrication and operation of microfluidic acoustophoretic chips using the microfluidic acoustophoresis technique and aptamer-modified microbeads that can be used for fast, efficient isolation of Gram-negative bacteria from a medium. Engineering Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration Hong Jin Choi1, Jong Hyun Lee2, Ok Chan Jeong1,2,3 1Department of Digital Anti-Aging Health Care, Inje University, 2Micro device Lab Co., Ltd, 3Department of Biomedical Engineering, Inje University The present protocol describes a pneumatic microfluidic platform that can be used for efficient microparticle concentration. Behavior Using a Virtual Reality Walking Simulator to Investigate Pedestrian Behavior Hyun Chae Chung1, Soon Ho Kim2, Gyoojae Choi3, Jong Won Kim4, Moo Young Choi2, Hui Li1 1Department of Sport and Exercise Sciences, Kunsan National University, 2Department of Physics and Astronomy, Seoul National University, 3School of Mechanical and Automotive Engineering, Kunsan National University, 4Department of Healthcare Information Technology, Inje University This protocol describes use of a walking simulator that serves as a safe and ecologically valid method to study pedestrian behavior in the presence of moving traffic. Bioengineering Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats In Gul Kim1, Yanru Wu2, Su A. Park3, Hana Cho1, Jung-Woog Shin2, Eun-Jae Chung1 1Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University, College of Medicine, 2Department of Biomedical Engineering, Inje University, Gimhae, 3Department of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and Materials Esophageal reconstruction is a challenging procedure, and development of a tissue-engineered esophagus that enables regeneration of esophageal mucosa and muscle and that can be implanted as an artificial graft is necessary. Here, we present our protocol to generate an artificial esophagus, including scaffold manufacturing, bioreactor cultivation, and various surgical techniques. Bioengineering A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons Se Hee Lee*1, Ho Won Lee*2, Da Som Kim2, Hyuck Gi Kwon3, Jong Hyun Lee3, Yang-Hoon Kim1, Ok Chan Jeong2,3, Ji-Young Ahn1 1College of Natural Sciences, Chungbuk National University, 2Department of Biomedical Engineering, Inje University, 3Institute of Digital Anti-Aging Healthcare, Inje University This work provides a method for the fabrication of droplet-based microfluidic platforms and the application of polyacrylamide microspheres for microsphere-PCR amplification. The microsphere-PCR method makes it possible to obtain single-stranded DNA amplicons without separating double-stranded DNA.