2 articles published in JoVE
Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats In Gul Kim1, Yanru Wu2, Su A. Park3, Hana Cho1, Jung-Woog Shin2, Eun-Jae Chung1 1Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University, College of Medicine, 2Department of Biomedical Engineering, Inje University, Gimhae, 3Department of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and Materials Esophageal reconstruction is a challenging procedure, and development of a tissue-engineered esophagus that enables regeneration of esophageal mucosa and muscle and that can be implanted as an artificial graft is necessary. Here, we present our protocol to generate an artificial esophagus, including scaffold manufacturing, bioreactor cultivation, and various surgical techniques.
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons Se Hee Lee*1, Ho Won Lee*2, Da Som Kim2, Hyuck Gi Kwon3, Jong Hyun Lee3, Yang-Hoon Kim1, Ok Chan Jeong2,3, Ji-Young Ahn1 1College of Natural Sciences, Chungbuk National University, 2Department of Biomedical Engineering, Inje University, 3Institute of Digital Anti-Aging Healthcare, Inje University This work provides a method for the fabrication of droplet-based microfluidic platforms and the application of polyacrylamide microspheres for microsphere-PCR amplification. The microsphere-PCR method makes it possible to obtain single-stranded DNA amplicons without separating double-stranded DNA.