HCTM Co. Ltd 1 article published in JoVE Engineering Development of a Nose-only Inhalation Toxicity Test Chamber That Provides Four Exposure Concentrations of Nano-sized Particles Jae-Seong Yi1,2, Ki-Soo Jeon2, Hee-Jung Kim2, Ki-Joon Jeon1, Il-Je Yu2 1Department of Environmental Engineering, Inha University, 2HCTM Co. Ltd A nose-only inhalation toxicity chamber capable of testing inhalation toxicity at four different exposure concentrations was designed and validated for flow field uniformity and cross-contamination between the exposure ports for each concentration. Here, we present a protocol to confirm that the designed chamber is effective for inhalation toxicity testing.