Asan Medical Institute of Convergence Science and Technology 1 article published in JoVE Biology A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells Jeong Hoon Lee1, Jeong Mi Ha1, Quynh Mai Ho1, Chae Hun Leem1,2,3 1Department of Physiology, University of Ulsan College of Medicine/Asan Medical Center, 2Asan Medical Center, 3Asan Medical Institute of Convergence Science and Technology Due to the spectral overlapping of the excitation and emission wavelengths of NADH and fura-2 analogs, the signal interference from both chemicals in live cells is unavoidable during quantitative measurement of [Ca2+]. Thus, a novel online correction method of NADH signal interference to measure [Ca2+] was developed.
