3 articles published in JoVE
Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus Anvar Sariev1,2, Jinho Chung1, Dajung Jung1,3, Farnaz Sharif1,2, Ju-Young Lee1,2, Soyoun Kim1, Sebastien Royer1,2 1Center for Functional Connectomics, Korea Institute of Science and Technology, 2Biomedical department, KIST school, University of Science and Technology, 3Department of Biological Sciences, Korea Advanced Institute of Science and Technology We describe the diverse steps to implant chronic silicon probes and to record place cells in mice that are running head-fixed on a cue-enriched treadmill apparatus.
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells Md Nazmul Huda1,2, Erdenedolgor Erdene-Ochir1,2, Cheol-Ho Pan1,2 1Systems Biotechnology Research Center, KIST Gangneung Institute of Natural Products, 2Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology This manuscript describes standard protocols for measuring tau hyperphosphorylation, measuring tau binding to microtubules, and localization of intracellular tau following drug treatments. These protocols can be used repetitively for screening drugs or other compounds that target tau hyperphosphorylation or microtubule binding.
High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode Mina Lee1,2, Dongwook Kim1,2, Hee-Sup Shin1,2, Ho-Geun Sung3, Jee Hyun Choi1,2 1Center for Neural Science , Korea Institute of Science and Technology (KIST), 2Department of Neuroscience, University of Science and Technology, 3Fab Service Department, Korea Advanced Nano Fab Center In this article, we described the surgery procedure and handling tips for implantation of ultra-thin polyimide-based microelectrode array (PBM-array) on the mouse skull for acquisition of high-density encephalography (EEG) in a mouse model.