University of Science and Technology (Korea)

10 articles published in JoVE

• Cancer Research

  
  Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells Jina Lee*^1,2, Joo-Eun Lee*³, Seil Kim^1,4,5, Dukjin Kang¹, Hee Min Yoo^{1 1}Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), ²College of Pharmacy, Chungnam National University (CNU), ³Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), ⁴Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), ⁵Department of Bio-Analysis Science, University of Science & Technology (UST) Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways.

• Medicine

  
  Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of Caenorhabditis elegans Tram Anh Ngoc Le¹, Baskar Selvaraj^1,2, Jae Wook Lee^1,2, Kyungsu Kang^{1,2 1}Gangneung Institute of Natural Products, Korea Institute of Science and Technology, ²Division of Bio-Medical Science & Technology, Korea University of Science and Technology (UST) This protocol describes how to measure intestinal permeability of Caenorhabditis elegans. This method is helpful for basic biological research on intestinal health related to the interaction between intestinal bacteria and their host and for screening to identify probiotic and chemical agents to cure leaky gut syndrome and inflammatory bowel diseases.

• Engineering

  
  Fabrication of Robust Nanoscale Contact between a Silver Nanowire Electrode and CdS Buffer Layer in Cu(In,Ga)Se₂ Thin-film Solar Cells Sangyeob Lee^1,2, Kyung Soo Cho², Soomin Song³, Kihwan Kim^3,4, Young-Joo Eo^3,4, Jae Ho Yun^3,4, Jihye Gwak^3,4, Choong-Heui Chung^{1,2 1}Department of Materials Science and Engineering, Hanbat National University, ²Department of Materials and Manufacturing Engineering, Hanbat National University, ³Photovoltaics Laboratory, Korea Institute of Energy Research, ⁴Renewable Energy Engineering, University of Science and Technology, Daejeon In this protocol, we describe the detailed experimental procedure for the fabrication of a robust nanoscale contact between a silver nanowire network and CdS buffer layer in a CIGS thin-film solar cell.

• Engineering

  
  An Efficient Method for Selective Desalination of Radioactive Iodine Anions by Using Gold Nanoparticles-Embedded Membrane Filter Ha Eun Shim*^1,2, Sajid Mushtaq*^1,3, Jongho Jeon^{1,3 1}Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, ²Department of Chemistry, Kyungpook National University, ³Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology An efficient method for the rapid and ion-selective desalination of radioactive iodine in several aqueous solutions is described by using gold nanoparticles-immobilized cellulose acetate membrane filters.

• Neuroscience

  
  Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus Anvar Sariev^1,2, Jinho Chung¹, Dajung Jung^1,3, Farnaz Sharif^1,2, Ju-Young Lee^1,2, Soyoun Kim¹, Sebastien Royer^{1,2 1}Center for Functional Connectomics, Korea Institute of Science and Technology, ²Biomedical department, KIST school, University of Science and Technology, ³Department of Biological Sciences, Korea Advanced Institute of Science and Technology We describe the diverse steps to implant chronic silicon probes and to record place cells in mice that are running head-fixed on a cue-enriched treadmill apparatus.

• Biochemistry

  
  Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells Md Nazmul Huda^1,2, Erdenedolgor Erdene-Ochir^1,2, Cheol-Ho Pan^{1,2 1}Systems Biotechnology Research Center, KIST Gangneung Institute of Natural Products, ²Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology This manuscript describes standard protocols for measuring tau hyperphosphorylation, measuring tau binding to microtubules, and localization of intracellular tau following drug treatments. These protocols can be used repetitively for screening drugs or other compounds that target tau hyperphosphorylation or microtubule binding.

• Chemistry

  
  An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a ¹²⁵I-labeled Azide Prosthetic Group Jongho Jeon^1,2, Ha Eun Shim¹, Sajid Mushtaq^1,2, Mi Hee Choi¹, Sang Hyun Park^1,2, Dae Seong Choi¹, Beom-Su Jang^{1,2 1}Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, ²Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology A detailed procedure for the synthesis of a ¹²⁵I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.

• Biochemistry

  
  Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer Haseong Kim*¹, Gui Hwan Han*¹, Yaoyao Fu¹, Jongsik Gam², Seung Goo Lee^{1,3 1}Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, ²College of Interdisciplinary & Creative Studies, Konyang University, ³Biosystems and Bioengineering Program, University of Science and Technology This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.

• Neuroscience

  
  Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits Hye Yun Kim^1,2, Dongkeun K. Lee¹, Bo-Ryehn Chung^1,3, Hyunjin V. Kim^1,4, YoungSoo Kim^{1,4 1}Center for Neuro-Medicine, Korea Institute of Science and Technology, ²Research Institute, GoshenBiotech, Inc., ³Department of Chemical and Biological Engineering, Princeton University, ⁴Biological Chemistry Program, Korea University of Science and Technology The amyloid-β (Aβ)-injected animal model enables the administration of a defined quantity and species of Aβ fragments and reduces individual differences within each study group. This protocol describes the intracerebroventricular (ICV) injection of Aβ without stereotactic instruments, enabling the production of Alzheimer-like behavioral abnormalities in normal mice.

• Neuroscience

  
  High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode Mina Lee^1,2, Dongwook Kim^1,2, Hee-Sup Shin^1,2, Ho-Geun Sung³, Jee Hyun Choi^{1,2 1}Center for Neural Science , Korea Institute of Science and Technology (KIST), ²Department of Neuroscience, University of Science and Technology, ³Fab Service Department, Korea Advanced Nano Fab Center In this article, we described the surgery procedure and handling tips for implantation of ultra-thin polyimide-based microelectrode array (PBM-array) on the mouse skull for acquisition of high-density encephalography (EEG) in a mouse model.