Universiti Sains Malaysia 3 articles published in JoVE Biology Eyestalk Ablation to Increase Ovarian Maturation in Mud Crabs Mohammad Asmat-Ullah1, Rusydi Rozaimi1, Hanafiah Fazhan1,2,3, Alexander Chong Shu-Chien2,4, Youji Wang5, Khor Waiho1,2,3 1Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 2Centre for Chemical Biology, Universiti Sains Malaysia, 3Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 4School of Biological Sciences, Universiti Sains Malaysia, 5International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University Two eyestalk ablation protocols (i.e., cauterization and surgery approaches) were performed on anesthetized female crabs. The eyestalk ablation of mud crabs hastened the ripening of ovaries without decreasing the survival rate. Medicine Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells Simone S. E. Nielsen1, Piotr Siupka1, Ana Georgian2, Jane E. Preston2, Andrea E. Tóth1, Siti R. Yusof2,3, N. Joan Abbott2, Morten S. Nielsen1 1Lundbeck Foundation Research Initiative on Brain Barriers and Drug Delivery, Department of Biomedicine, Aarhus University, 2 The aim of the protocol is to present an optimized procedure for the establishment of an in vitro blood-brain barrier (BBB) model based on primary porcine brain endothelial cells (pBECs). The model shows high reproducibility, high tightness, and is suitable for studies of transport and intracellular trafficking in drug discovery. Immunology and Infection Peptide-based Identification of Functional Motifs and their Binding Partners Martin N. Shelton1,2, Ming Bo Huang1, Syed Ali1,3, Kateena Johnson1, William Roth1, Michael Powell1, Vincent Bond1 1Department of Microbiology, Biochemistry, & Immunology, Morehouse School of Medicine, 2Institute for Systems Biology, 3Advanced Medical & Dental Institute, Universiti Sains Malaysia Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.
