2 articles published in JoVE
Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction Dmitry V. Samigullin1,2,3, Eduard F. Khaziev1,2,3, Nikita V. Zhilyakov1,2, Ellya A. Bukharaeva1,2, Eugeny E. Nikolsky1,2,4 1Laboratory of Biophysics of Synaptic Processes, Kazan Scientific Centre, Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, 2Open Laboratory of Neuropharmacology, Kazan Federal University, 3Department of Radiophotonics and Microwave Technologies, A.N. Tupolev Kazan National Research Technical University, 4Department of Medical and Biological Physics, Kazan State Medical University Here, we describe a method for loading a calcium-sensitive dye through the frog nerve stump into the nerve endings. We also present a protocol for the recording and analysis of fast calcium transients in the peripheral nerve endings.
Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy Gabriele Di Sante1, Mathew C. Casimiro1, Timothy G. Pestell2, Richard G. Pestell1,2,3 1Department of Cancer Biology, Thomas Jefferson University, SKCC, 2Department of Medical Oncology, Thomas Jefferson University, SKCC, 3Kazan Federal University Herein, we describe in detail a time-lapse video microscopy approach to measuring the temporal recruitment of EYFP-Parkin during the selective removal of damaged mitochondria. This dynamic process of EYFP-Parkin-dependent removal of damaged mitochondria can be used as an indicator of cellular health under different experimental conditions.