2 articles published in JoVE
Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung Ivan V. Maslov1, Andrey O. Bogorodskiy1, Mariia V. Pavelchenko1, Ilia O. Zykov1, Natalya I. Troyanova2, Valentin I. Borshchevskiy1,3, Marina A. Shevchenko2 1Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 2Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 3Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich We describe the method for quantitative analysis of the distribution of Aspergillus fumigatus conidia (3 µm in size) in the airways of mice. The method also can be used for the analysis of microparticles and nanoparticle agglomerate distribution in the airways in various pathological condition models.
Imaging of Extracellular Vesicles by Atomic Force Microscopy Mikhail Skliar1,2, Vasiliy S. Chernyshev3,4 1Department of Chemical Engineering, University of Utah, 2The Nano Institute of Utah, University of Utah, 3Center for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, 4Biopharmaceutical Cluster 'Northern', Moscow Institute of Physics and Technology A step-by-step procedure is described for label-free immobilization of exosomes and extracellular vesicles from liquid samples and their imaging by atomic force microscopy (AFM). The AFM images are used to estimate the size of the vesicles in the solution and characterize other biophysical properties.