2 articles published in JoVE
Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction Dmitry V. Samigullin1,2,3, Eduard F. Khaziev1,2,3, Nikita V. Zhilyakov1,2, Ellya A. Bukharaeva1,2, Eugeny E. Nikolsky1,2,4 1Laboratory of Biophysics of Synaptic Processes, Kazan Scientific Centre, Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, 2Open Laboratory of Neuropharmacology, Kazan Federal University, 3Department of Radiophotonics and Microwave Technologies, A.N. Tupolev Kazan National Research Technical University, 4Department of Medical and Biological Physics, Kazan State Medical University Here, we describe a method for loading a calcium-sensitive dye through the frog nerve stump into the nerve endings. We also present a protocol for the recording and analysis of fast calcium transients in the peripheral nerve endings.
Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo Benjamen A. Filas1, Victor D. Varner1, Dmitry A. Voronov1,2, Larry A. Taber1,3 1Department of Biomedical Engineering, Washington University, 2Institute for Information Transmission Problems, Russian Academy of Sciences, 3Department of Mechanical Engineering and Materials Science, Washington University This article describes surface labeling and ex ovo tissue culture in the early chick embryo. Techniques amenable to time-lapse bright field, fluorescence, and optical coherence tomography imaging are presented. Tracking surface labels with high spatiotemporal resolution enables kinematic quantities such as morphogenetic strains (deformations) to be calculated in both two and three dimensions.