King Saud University 7 articles published in JoVE Biology A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage Yunhee Ji*1, Mahsa Karbaschi*2, Abdulhadi Abdulwahed*3, Natalia S. Quinete4, Mark D. Evans5, Marcus S. Cooke1 1Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences, University of South Florida, 2Cepheid (Danaher Corp.), US Technical Operations, 3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, 4Department of Chemistry and Biochemistry, Institute of Environment, Florida International University, 5Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences, De Montfort University The comet assay is a popular means of detecting DNA damage. This study describes an approach to running slides in representative variants of the comet assay. This approach significantly increased the number of samples while decreasing assay run-time, the number of slide manipulations, and the risk of damage to gels. Bioengineering Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels Abdulaziz S. Fakhouri1,2,3, Jennifer L. Leight1,2 1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University Here, a protocol is presented for encapsulating and culturing cells in poly(ethylene glycol) (PEG) hydrogels functionalized with a fluorogenic matrix metalloproteinase (MMP)-degradable peptide. Cellular MMP and metabolic activity are measured directly from the hydrogel cultures using a standard microplate reader. Bioengineering Exploring the Potential of Mesenchymal Stem Cell Sheet on The Development of Hepatocellular Carcinoma In Vivo Alaa T Alshareeda1, Batla Alsowayan1, Abdullah Almubarak2, Ayidah Alghuwainem1, Yasser Alshawakir2, Mohammed Alahmed2 1Stem Cell and Regenerative Medicine Department, King Abdullah International Medical Research Centre, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, 2College of Medicine, Experimental Surgery and Animal Laboratory, King Saud University Here, we present a protocol to develop an in vivo cancer model using cell sheet technology. Such a model could be very useful for the evaluation of anticancer therapeutics. Chemistry Chemical Synthesis of Porous Barium Titanate Thin Film and Thermal Stabilization of Ferroelectric Phase by Porosity-Induced Strain Norihiro Suzuki1,2, Minoru Osada3, Motasim Billah3,4, Yoshio Bando3,4, Yusuke Yamauchi5,6, Shahriar A. Hossain3,4 1Research Institute for Science and Technology (RIST), Tokyo University of Science (TUS), 2International Center for Young Scientists (ICYS), National Institute for Materials Science (NIMS), 3International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 4Australian Institute for Innovative Materials (AIIM), University of Wollongong, 5School of Chemical Engineering, Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, 6Department of Plant & Environmental New Resources, Kyung Hee University Here, we present a protocol for the synthesis of porous barium titanate (BaTiO3) thin film by a surfactant-assisted sol-gel method, in which self-assembled amphipathic surfactant micelles are used as an organic template. Bioengineering Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions Tzu-Cheng Sung*1, Hsing-Fen Li*1, Akon Higuchi1,2, Qing-Dong Ling3,4, Jia-Sin Yang1, Yeh-Chia Tseng1, Chih-Hsien Pan Pan1, Abdullah A. Alarfaj2, Murugan A. Munusamy2, Suresh Kumar5, Shih-Tien Hsu6, Kadarkarai Murugan7,8 1Department of Chemical and Materials Engineering, National Central University, 2Department of Botany and Microbiology, King Saud University, 3Cathay Medical Research Institute, Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics, National Central University, 5Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, 6Department of Internal Medicine, Taiwan Landseed Hospital, 7Department of Zoology, Bharathiar University, 8Thiruvalluvar University A protocol is presented to prepare polyvinyl alcohol-co-itaconic acid hydrogels with varying stiffness, which were grafted with and without oligopeptides, to investigate the effect of the stiffness of biomaterials on the differentiation and proliferation of stem cells. The stiffness of the hydrogels was controlled by the crosslinking time. Bioengineering 20 mJ, 1 ps Yb:YAG Thin-disk Regenerative Amplifier Ayman Alismail1,2, Haochuan Wang1,3, Jonathan Brons3, Hanieh Fattahi1,3 1Department of Physics, Ludwig Maximilian University of Munich, 2Physics and Astronomy Department, King Saud University, 3Max Planck Institute of Quantum Optics A protocol for the operation of a high-energy, high-power optical parametric chirped pulse amplifier pump source based on an Yb:YAG thin-disk regenerative amplifier is presented here. Biology The Slice Culture Method for Following Development of Tooth Germs In Explant Culture Sarah A. Alfaqeeh1,2, Abigail S. Tucker1 1 Here we detail a method to culture tooth germs in mandible slices using a tissue chopper. This method allows unique access to the tooth during development, providing excellent opportunity for manipulation and lineage tracing, not available using more traditional culture methods.
