National Heart Centre Singapore 1 article published in JoVE Engineering In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart Sindhu Ramesh*1, Anamika Singh*1, Dasan M. Cibi1, Derek J. Hausenloy1,2,3, Manvendra K. Singh1,2 1Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical School, 2National Heart Research Institute Singapore, National Heart Centre Singapore, 3The Hatter Cardiovascular Insititute, University College London The epicardium is an essential source of multipotent cardiovascular progenitor cells and paracrine factors that are required for cardiovascular development and regeneration. We describe here a method to culture mouse embryonic epicardial cells.
