Singapore Immunology Network 2 articles published in JoVE Immunology and Infection Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection Zhe Zhang Ryan Lew*1, Jing Liu*1, Hsiao Hui Ong1, Vivian Jiayi Tan2, Annika Luukkainen3, Yew Kwang Ong1,4, Mark Thong1,4, Kia Joo Puan5, Vincent Tak Kwong Chow2,6, Kai Sen Tan1,2,6, De Yun Wang1,6 1Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 3Haartman Institute, Medicum, University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, 5Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore This protocol details an investigation of the early interactions between virally infected nasal epithelial cells and innate cell activation. Individual subsets of immune cells can be distinguished based on their activation in response to viral infections. They can then be further investigated to determine their effects on early antiviral responses. Immunology and Infection Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes Wen Jing Sim1, Frano Malinarich1, Anna-Marie Fairhurst2, John Edward Connolly1,3 1Translational Immunology, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 2Singapore Immunology Network, 3Institute of Biomedical Studies, Baylor University Immature dendritic cells can be selectively differentiated into tolerogenic or mature dendritic cells to regulate the balance between immunity and tolerance. This work presents a means to generate from immature monocyte derived dendritic cells (moDCs), in vitro tolerogenic and mature moDCs that differ in metabolic phenotypes.
