National Cancer Centre Singapore 1 article published in JoVE Cancer Research Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods Jiajun Yap1, Jimin Yuan1, Zi Heng Tee1, Xuchao Huang1, Wan Hwa Ng1, Jiancheng Hu1,2 1The Laboratory of Cancer Signaling, Division of Cellular and Molecular Research, National Cancer Centre Singapore, 2The Cancer and Stem Cell Program, Duke-NUS Medical School In this article, we presented a set of practical and feasible methods for characterizing disease-related mutants of RAF family kinases, which include in vitro kinase assay, RAF co-activation assay, and complementary split luciferase assay.
