3 articles published in JoVE
Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation Sirada Srihirun1,2, Alan N. Schechter2, Barbora Piknova2 1Department of Pharmacology, Faculty of Dentistry, Mahidol University, 2Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health Here, we present a protocol to address the potential use of platelets as a highly sensitive nitric oxide sensor in blood. It describes initial platelet preparation and the use of nitrite and deoxygenated red blood cells as nitric oxide generators.
Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model Panithi Sukho1,2,3, Geesien S.A. Boersema4, Nicole Kops5, Johan F. Lange4, Jolle Kirpensteijn1,6, Jan Willem Hesselink1, Yvonne M. Bastiaansen-Jenniskens5, Femke Verseijden1,5 1Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 2Department of Otorhinolaryngology, Erasmus MC University Medical Center, 3Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, 4Department of Surgery, Erasmus MC University Medical Center, 5Department of Orthopaedics, Erasmus MC University Medical Center, 6 The preparation and transplantation of adipose tissue derived stem cell (ASC) sheets onto insufficiently sutured colorectal anastomoses in a rat model is presented. This novel application shows that ASC sheets can reduce colorectal anastomosis leakage.
Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages Kuldip Sidhu1, Jaemin Kim1, Methichit Chayosumrit2, Sophia Dean1, Perminder Sachdev3 1Stem Cell Lab, School of Psychiatry, Faculty of Medicine, The University of New South Wales, 2Siriraj Center of Excellence for Stem cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 3Neuropsychiatric Institute, Prince of Wales Hospital We have optimized a microencapsulation technique as an effective 3D platform for propagation and differentiation of embryonic stem cells to endoderm and dopaminergic (DA) neurons. It also provides an opportunity for immune-isolation of cells from the host during transplantation. This platform can be adapted for other cell types.