2 articles published in JoVE
Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model Panithi Sukho1,2,3, Geesien S.A. Boersema4, Nicole Kops5, Johan F. Lange4, Jolle Kirpensteijn1,6, Jan Willem Hesselink1, Yvonne M. Bastiaansen-Jenniskens5, Femke Verseijden1,5 1Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 2Department of Otorhinolaryngology, Erasmus MC University Medical Center, 3Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, 4Department of Surgery, Erasmus MC University Medical Center, 5Department of Orthopaedics, Erasmus MC University Medical Center, 6 The preparation and transplantation of adipose tissue derived stem cell (ASC) sheets onto insufficiently sutured colorectal anastomoses in a rat model is presented. This novel application shows that ASC sheets can reduce colorectal anastomosis leakage.
Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages Kuldip Sidhu1, Jaemin Kim1, Methichit Chayosumrit2, Sophia Dean1, Perminder Sachdev3 1Stem Cell Lab, School of Psychiatry, Faculty of Medicine, The University of New South Wales, 2Siriraj Center of Excellence for Stem cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 3Neuropsychiatric Institute, Prince of Wales Hospital We have optimized a microencapsulation technique as an effective 3D platform for propagation and differentiation of embryonic stem cells to endoderm and dopaminergic (DA) neurons. It also provides an opportunity for immune-isolation of cells from the host during transplantation. This platform can be adapted for other cell types.