Chulabhorn Graduate Institute 1 article published in JoVE Bioengineering CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells Jing Ge*1, Somsak Prasongtanakij*2, David K. Wood3, David M. Weingeist1, Jessica Fessler1, Panida Navasummrit2, Mathuros Ruchirawat2, Bevin P. Engelward1 1Department of Biological Engineering, Massachusetts Institute of Technology, 2Environmental Toxicology, Chulabhorn Graduate Institute, 3Department of Biomedical Engineering, University of Minnesota We describe here a platform that allows comet assay detection of DNA damage with unprecedented throughput. The device patterns mammalian cells into a microarray and enables parallel processing of 96 samples. The approach facilitates analysis of base level DNA damage, exposure-induced DNA damage and DNA repair kinetics.
