National Tsing Hua University 11 articles published in JoVE Engineering A Versatile Kit Based on Digital Microfluidics Droplet Actuation for Science Education Yu-Hao Guo1, Chih-Hsien Lee1, Ya-Tang Yang1 1Electrical Engineering, National Tsing Hua University We describe an educational kit that allows users to execute multiple experiments and gain hands-on experience on digital microfluidics. Behavior Dissociation of the Confounding Influences of Expectancy and Integrative Difficulty Residing in Anomalous Sentences in Event-related Potential Studies Yunlong Huang1, Minghu Jiang1, Qian Guo2, Yuling Wang1, Fan-Pei Gloria Yang3 1Center for Psychology and Cognitive Science, School of Humanities, Tsinghua University, 2Department of Foreign Languages and Literatures, School of Humanities, Tsinghua University, 3Center for Cognition and Mind Sciences, Lab for Language and Cognitive Neurology, National TsingHua University We present a protocol to dissociate the intertwining factors of integrative difficulty and unexpectedness in semantically anomalous sentences by applying multiple repetitions to enhance participant's expectancy for anomalous sentences. The dissociation helps to investigate the major contributor of elicited event-related potentials (ERP) effects such as N400 in language studies. Bioengineering Trapping of Micro Particles in Nanoplasmonic Optical Lattice Dinesh Bhalothia1, Ya-Tang Yang1 1Electrical Engineering, National Tsing Hua University We describe a procedure to optically trap micro-particles in nanoplasmonic optical lattice. Bioengineering Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel Hsin-Yi Hsieh1, Chiao-Wen Chu2, Ming-Hsuan Chiu1, Shueh-Yao Chu2, Tsu-Wei Huang2, Fan-Gang Tseng1,2 1Institute of NanoEngineering and MicroSystems, National Tsing Hua University, 2Department of Engineering and System, National Tsing Hua University This article introduces a simple approach to providing non-continuous gradient static strains on a concentric cell-laden hydrogel to regulate cell alignment for tissue engineering. Engineering Blue-hazard-free Candlelight OLED Jwo-Huei Jou1, Meenu Singh1, Yu-Ting Su1, Shih-Hao Liu1, Zhe-Kai He1 1Department of Materials Science and Engineering, National Tsing Hua University We present a protocol for the fabrication of a blue-hazard-free candlelight organic light emitting diode (OLED) for eye protection and melatonin secretion. Genetics Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection Po C. Liu1, Yi T. Lee1, Chun Y. Wang1, Ya-Tang Yang1 1Electrical Engineering, National Tsing Hua University We describe a low cost, configurable morbidostat that enables the characterization of antibiotic drug resistance by dynamically adjusting the drug concentration. The device can be integrated with a multiplexed microfluidic platform. The approach can be scaled up for laboratory antibiotic drug resistance studies. Bioengineering Fabricating Cotton Analytical Devices Shang-Chi Lin*1, Min-Yen Hsu*2, Chen-Meng Kuan1, Fan-Gang Tseng3, Chao-Min Cheng4 1Institute of Nanoengineering and Microsystems, National Tsing Hua University, 2Department of Ophthalmology, Taichung Veterans General Hospital, 3Department of Engineering and System Science, National Tsing Hua University, 4Institute of Biomedical Engineering, National Tsing Hua University To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen. Bioengineering Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples Tsung-Ting Shih*1, I-Hsiang Hsu*2, Ping-Hung Chen1, Shun-Niang Chen1, Sheng-Hao Tseng1, Ming-Jay Deng3, Yang-Wei Lin4, Yuh-Chang Sun1 1Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 2Center for Measurement Standards, Industrial Technology Research Institute, 3National Synchrotron Radiation Research Center, 4Department of Chemistry, National Changhua University of Education The fabrication protocol of a dipole-assisted solid phase extraction microchip for the trace metal analysis is presented. Immunology and Infection One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode Ting-Yu Kuo1, Zhi-Wei Hong1, Chung-Che Tsai1, Yu-Chi Yang1, Hua-Wen Fu1,2 1Institute of Molecular and Cellular Biology, National Tsing Hua University, 2Department of Life Science, National Tsing Hua University A high yield method for one-step negative purification of recombinant Helicobacter pylori neutrophil-activating protein (HP-NAP) overexpressed in Escherichia coli by using diethylaminoethyl resins in batch mode is described. HP-NAP purified by this method is beneficial for the development of vaccines, drugs, or diagnostics for H. pylori-associated diseases. Bioengineering A Microfluidic Platform for High-throughput Single-cell Isolation and Culture Ching-Hui Lin1,2, Hao-Chen Chang1,2, Chia-Hsien Hsu1,2,3 1Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Taiwan, 2Tissue Engineering and Regenerative Medicine, National Chung Hsing University, 3Institute of NanoEngineering and MicroSystems, National Tsing Hua University Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture. Bioengineering Paper-based Devices for Isolation and Characterization of Extracellular Vesicles Chihchen Chen1, Bo-Ren Lin1, Min-Yen Hsu2, Chao-Min Cheng1 1Institute of Nanoengineering and Microsystems, National Tsing Hua University, 2Taichung Veterans General Hospital This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 μl serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited volumes.
