Linkou Chang Gung Memorial Hospital 5 articles published in JoVE Medicine Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium Chun Hsiung*1,2, Yu-Ting Liu*1,2, Chen-Ying Su3, Chung-Chuan Hsiung4,5, Kuo-Hsuan Hung2,6,7, Lung-Kun Yeh2,6 1Department of Education, Chang Gung Memorial Hospital Linkou, 2School of Medicine, Chang Gung University, 3Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 4Cell Therapy Center, Tri-Service General Hospital, 5Department of General Surgery, Bo-Ai Clinic, 6Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, 7Institute of Clinical Medicine, National Yang Ming Chiao Tung University This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo. Medicine Development of a Novel Task-oriented Rehabilitation Program using a Bimanual Exoskeleton Robotic Hand Yi-Mei Chen1, Szu-Shen Lai1, Yu-Cheng Pei2,3,4,5, Chia-Ju Hsieh1, Wei-Han Chang1 1Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Taoyuan, 2Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Linkou, 3School of Medicine, Chang Gung University, 4Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, 5Healthy Aging Research Center, Chang Gung University This study reports the development of a novel robot-assisted task-oriented program for hand rehabilitation. The developmental process consists of experiments using both healthy subjects and subjects who have had a stroke and suffered from subsequent motor control dysfunction. Neuroscience Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release Ya-Tin Lin1, Jin-Chung Chen1,2,3 1Graduate Institute of Biomedical Sciences, Department of Physiology and Pharmacology, Chang Gung University, 2Healthy Aging Research Center, Chang Gung University, 3Neuroscience Research Center, Chang Gung Memorial Hospital Dorsal root ganglia (DRG) primary cultures are frequently used to study physiological functions or pathology-related events in sensory neurons. Here, we demonstrate the use of lumbar DRG cultures to detect the release of neurotransmitters after neuropeptide FF receptor type 2 stimulation with a selective agonist. Medicine Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery Shao-Chieh Chiu1, Shih-Ting Hsu1, Chiun-Wei Huang1, Wu-Chung Shen2, Shin-Lei Peng2 1Center for Advanced Molecular Imaging and Translation, Chang Gung Memorial Hospital, 2Department of Biomedical Imaging and Radiological Science, China Medical University The overall goal of this procedure is to measure the blood flow in the rat common carotid artery by using noninvasive phase contrast magnetic resonance imaging. Neuroscience A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood Chia-Hsiang Chen1,2, Yu-Syuan Chang1 1Department of Psychiatry, Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences, Chang Gung University We ectopically expressed NR1 subunit of NMDA receptor tagged with green fluorescent protein in human embryonic cells (HEK293) as antigen to detect autoantibodies against NMDA receptor in the blood of patients suspected with autoimmune encephalitis. This simple method may be suitable for screening purposes in clinical settings.
