3 articles published in JoVE
Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release Ya-Tin Lin1, Jin-Chung Chen1,2,3 1Graduate Institute of Biomedical Sciences, Department of Physiology and Pharmacology, Chang Gung University, 2Healthy Aging Research Center, Chang Gung University, 3Neuroscience Research Center, Chang Gung Memorial Hospital Dorsal root ganglia (DRG) primary cultures are frequently used to study physiological functions or pathology-related events in sensory neurons. Here, we demonstrate the use of lumbar DRG cultures to detect the release of neurotransmitters after neuropeptide FF receptor type 2 stimulation with a selective agonist.
Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery Shao-Chieh Chiu1, Shih-Ting Hsu1, Chiun-Wei Huang1, Wu-Chung Shen2, Shin-Lei Peng2 1Center for Advanced Molecular Imaging and Translation, Chang Gung Memorial Hospital, 2Department of Biomedical Imaging and Radiological Science, China Medical University The overall goal of this procedure is to measure the blood flow in the rat common carotid artery by using noninvasive phase contrast magnetic resonance imaging.
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood Chia-Hsiang Chen1,2, Yu-Syuan Chang1 1Department of Psychiatry, Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences, Chang Gung University We ectopically expressed NR1 subunit of NMDA receptor tagged with green fluorescent protein in human embryonic cells (HEK293) as antigen to detect autoantibodies against NMDA receptor in the blood of patients suspected with autoimmune encephalitis. This simple method may be suitable for screening purposes in clinical settings.