National Taiwan University of Science and Technology 5 articles published in JoVE Bioengineering Wet-spinning-based Molding Process of Gelatin for Tissue Regeneration Chia-Yu Wang1, Dewi Sartika2, Ding-Han Wang3, Po-Da Hong1, Juin-Hong Cherng2,4,5, Shu-Jen Chang2,6, Cheng-Che Liu7, Yi-Wen Wang4, Sheng-Tang Wu8 1Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 2Laboratory of Adult Stem Cell and Tissue Regeneration, National Defense Medical Center, 3School of Dentistry, National Yang-Ming University, 4Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, 5Department of Gerontological Health Care, National Taipei University of Nursing and Health Sciences, 6Division of Rheumatology/Immunology/Allergy, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, 7Department of Physiology and Biophysics, Graduate Institute of Physiology, National Defense Medical Center, 8Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center We developed and describe a protocol based on the wet spinning concept, for the construction of gelatin-based biomaterials used for the application of tissue engineering. Chemistry Synthesizing Sodium Tungstate and Sodium Molybdate Microcapsules via Bacterial Mineral Excretion Pao-Hung Lin1, Ying-Tang Huang2, Fu-Wen Lin3 1Department of Electronic and Computer Engineering, National Taiwan University of Science and Technology, 2Department of Marine Biotechnology, National Kaohsiung Marine University, 3Institute of Applied English, National Taiwan Ocean University This work presents a protocol for manufacture sodium tungstate and sodium molybdate microcapsules via bacteria and their corresponding nanoparticles. Developmental Biology Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues Yu-Jen Chen1,2, Hui-Yu Liu2, Yun-Tsui Chang2, Ying-Hung Cheng2, Harry J. Mersmann2, Wen-Hung Kuo3, Shih-Torng Ding1,2 1Institute of Biotechnology, National Taiwan University, 2Department of Animal Science and Technology, National Taiwan University, 3Department of Surgery, National Taiwan University Hospital and College of Medicine This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies. Engineering Ohmic Contact Fabrication Using a Focused-ion Beam Technique and Electrical Characterization for Layer Semiconductor Nanostructures Ruei-San Chen1, Chih-Che Tang2, Wei-Chu Shen2, Ying-Sheng Huang2 1Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, 2Department of Electronic Engineering, National Taiwan University of Science and Technology We describe the approaches for the device fabrication and electrical characterization of molybdenum diselenide (MoSe2) layer semiconductor nanostructures with different thicknesses. In addition, the fabrication of ohmic contacts for MoSe2-layer nanocrystals by the focused-ion beam deposition method using platinum (Pt) as a contact metal is described. Bioengineering Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique Chen-li Sun1, Tzu-hsun Hsiao2 1Department of Mechanical Engineering, National Taiwan University, 2Department of Mechanical Engineering, National Taiwan University of Science and Technology Herein, we describe a procedure that employs microscale schlieren technique to measure mixing inhomogeneity in a microfluidic device. Through calibration, distribution of concentration gradient can be derived from the micro-schlieren image.
