Chang Gung Memorial Hospital Linkou Medical Center and Chang Gung University College of Medicine 3 articles published in JoVE Medicine Genetic Analysis of Hereditary Transthyretin Ala97Ser Related Amyloidosis Hui-Ching Hsu1, Ming-Feng Liao2, Jung-Lung Hsu2, Yun-Lin Lee2, Long-Sun Ro2 1Department of Traditional Chinese Medicine, Division of Chinese Acupuncture and Traumatology, Chang Gung Memorial Hospital Linkou Medical Center and Chang Gung University College of Medicine, 2Department of Neurology, Chang Gung Memorial Hospital Linkou Medical Center and Chang Gung University College of Medicine Here, we present a protocol to confirm the presence of point mutation for the diagnosis of hereditary transthyretin amyloidosis, using Ala97Ser, the most common endemic mutation in Taiwan, as an example. Biochemistry A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues Chao-Yung Wang1, Mei-Hsiu Lin1, Hui-Ting Su1 1Department of Cardiology, Chang Gung Memorial Hospital, Chang Gung University College of Medicine A modified northern blotting method for measuring N6-methyladenosine (m6A) modifications in RNA is described. The current method can detect modifications in diverse RNAs and controls under various experimental designs. Medicine Orthotopic Hind Limb Transplantation in the Mouse Georg J. Furtmüller*1, Byoungchol Oh*1, Johanna Grahammer*2, Cheng-Hung Lin3, Robert Sucher4, Madeline L. Fryer1, Giorgio Raimondi1, W.P. Andrew Lee1, Gerald Brandacher1 1Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, Johns Hopkins University School of Medicine, 2Department of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, 3Center for Vascularized Composite Allotransplantation, Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital and School of Medicine, 4Department of General, Visceral and Transplant Surgery, Charite Berlin This novel model for orthotopic hind limb transplantation in the mouse, applying a non-suture cuff technique for super-microvascular anastomosis, provides a powerful tool for in vivo mechanistic immunological research related to vascularized composite allotransplantation (VCA).
