2 articles published in JoVE
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis Kang-Yi Su*1,2, Steven D. Goodman*3, Hung-Ming Lai1, Rong-Syuan Yen1, Wei-Yao Hu1, Wern-Cherng Cheng2, Liang-In Lin1,2, Ya-Chien Yang1,2, Woei-Horng Fang1,2 1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University A non-labeled, non-radio-isotopic method for DNA polymerase proofreading and a DNA repair assay was developed by using high-resolution MALDI-TOF mass spectrometry and a single nucleotide extension strategy. The assay proved to be very specific, simple, rapid, and easy to perform for proofreading and repair patches shorter than 9-nucleotides.
In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition Wei-Ting Ho1,2, Chien-Chia Su3, Jung-Shen Chang3, Shu-Wen Chang1, Fung-Rong Hu3, Tzuu-Shuh Jou2,4, I-Jong Wang3,4 1Department of Ophthalmology, Far Eastern Memorial Hospital, 2Institute of Clinical Medicine, National Taiwan University, 3Department of Ophthalmology, National Taiwan University Hospital, 4College of Medicine, National Taiwan University A primary culture of bovine corneal endothelial cells was used to investigate the mechanism of corneal endothelial-mesenchymal transition. Furthermore, a rat corneal endothelium cryoinjury model was used to demonstrate corneal endothelial-mesenchymal transition in vivo.