National Chung Hsing University 8 articles published in JoVE Biology Assessment of Aphidicidal Effect of Entomopathogenic Fungi against Parthenogenetic Insect, Mustard Aphid, Lipaphis erysimi (Kalt.) Cheng-Ju Yang1, Yu-Shin Nai1 1Department of Entomology, National Chung Hsing University This protocol presents an optimized detached-leaf bioassay system for evaluating the effectiveness of entomopathogenic fungi (EPF) against the mustard aphid (Lipaphis erysimi (Kalt.)), a parthenogenetic insect. The method outlines the data collection process during Petri dish experiments, enabling researchers to consistently measure the virulence of EPF against mustard aphids and other parthenogenetic insects. Bioengineering Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate Chien-Wei Cheng1, Shwu-Yuan Lee2, Tang-Yu Chen1, Jeu-Ming P. Yuann1, Chi-Ming Chiu1, Shiuh-Tsuen Huang3,4, Ji-Yuan Liang1 1Department of Biotechnology, Ming Chuan University, 2Department of Tourism and Leisure, Hsing Wu University, 3Department of Science Education and Application, National Taichung University of Education, 4Department of Soil and Environmental Sciences, National Chung Hsing University Here, we present a protocol to inactivate pathogenic bacteria with reactive oxygen species produced during photolysis of flavin mononucleotide (FMN) under blue and violet light irradiation of low intensity. FMN photolysis is demonstrated to be a simple and safe method for sanitary processes. Biology Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests Yao-Chia Liu1, Nian-Tong Ni1, Ju-Chun Chang1, Yi-Hsuan Li1, Mi Rong Lee2, Jae Su Kim2,3, Yu-Shin Nai1 1Department of Entomology, National Chung Hsing University, 2Department of Agricultural biology, Jeonbuk National University, 3Department of Agricultural Convergence Technology, Jeonbuk National University Here we present a protocol based on the mealworm (Tenebrio molitor)-bait system that was used for isolating and selecting entomopathogenic fungi (EPF) from soil samples. An effective conidia number (ECN) formula is used to select high stress tolerant EPF based on physiological characteristics for pest microbial control in the field. Bioengineering Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip Cheng-Kun He1,2, Ya-Wen Chen3, Ssu-Han Wang3, Chia-Hsien Hsu1,2 1Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, 2Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, 3National Institute of Cancer Research, National Health Research Institutes This report describes a microfluidic chip-based method to set up a single cell culture experiment in which high-efficiency pairing and microscopic analysis of multiple single cells can be achieved. Environment Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues Wen-Ta Li1, Bang-Yeh Liou1, Wei-Cheng Yang2, Meng-Hsien Chen3, Hui-Wen Chang1,2, Hue-Ying Chiou4, Victor Fei Pang1,2, Chian-Ren Jeng1,2 1Graduate Institute of Molecular and Comparative Pathobiology, National Taiwan University, 2School of Veterinary Medicine, National Taiwan University, 3Department of Oceanography and Asia-Pacific Ocean Research Center, National Sun Yat-sen University, 4Graduate Institute of Veterinary Pathobiology, National Chung Hsing University A protocol is presented to localize Ag in cetacean liver and kidney tissues by autometallography. Furthermore, a new assay, named the cetacean histological Ag assay (CHAA) is developed to estimate the Ag concentrations in those tissues. Engineering Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics Mon-Shu Ho1, Chih-Pong Huang2, Jyun-Hwei Tsai3, Che-Fu Chou1, Wen-Jay Lee3 1Department of Physics and Institute of Nanoscience, National Chung Hsing University, 2Metallurgy Section, Materials & Electro-Optics Research Division, National Chung-Shan Institute of Science and Technology, 3National Center for High-Performance Computing This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation. Bioengineering A Microfluidic Platform for High-throughput Single-cell Isolation and Culture Ching-Hui Lin1,2, Hao-Chen Chang1,2, Chia-Hsien Hsu1,2,3 1Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Taiwan, 2Tissue Engineering and Regenerative Medicine, National Chung Hsing University, 3Institute of NanoEngineering and MicroSystems, National Tsing Hua University Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture. Bioengineering Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip Hsien-San Hou1, Hui-Fang Chang1, Ji-Yen Cheng1,2,3,4,5 1Research Center for Applied Sciences, Academia Sinica, 2Institute of Biophotonics, National Yang-Ming University, 3Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, 4Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, 5Ph.D. Program in Microbial Genomics, National Chung Hsing University Many microfluidic devices have been developed for use in the study of electrotaxis. Yet, none of these chips allows the efficient study of the simultaneous chemical and electric-field (EF) effects on cells. We developed a polymethylmethacrylate-based device that offers better-controlled coexisting EF and chemical stimulation for use in electrotaxis research.
