Fu Jen Catholic University 6 articles published in JoVE Medicine Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research Hui-Chun Ku1, Cheng-Wei Huang1, Shih Yi Lee2,3 1Department of Life Science, Fu Jen Catholic University, 2MacKay Junior College of Medicine, Nursing, and Management, 3Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, MacKay Memorial Hospital This study established a protocol focusing on the technical refinement of a mouse model of bilateral renal ischemia-reperfusion for acute kidney injury research. Medicine Left Anterior Descending Coronary Artery Ligation for Ischemia-Reperfusion Research: Model Improvement via Technical Modifications and Quality Control Hui-Chun Ku1, Ding-Kuo Chien2,3,4,5, Chuan-Lei Chao5,6, Shih-Yi Lee5,7 1Department of Life Science, Fu Jen Catholic University, 2Department of Medicine, MacKay Medical College, 3Department of Emergency Medicine, MacKay Memorial Hospital, 4Graduate Institute of Injury Prevention and Control, Taipei Medical University, 5MacKay Junior College of Medicine, Nursing, and Management, 6Division of Cardiology, Department of Internal Medicine, Taitung Mackay Memorial Hospital, 7Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, MacKay Memorial Hospital Herein, we present a protocol focusing on the quality control of the left anterior descending coronary artery ligation by technically modifying the traditional procedure in rats for acute myocardial ischemia-reperfusion research. Immunology and Infection Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans Jia-Horung Hung*1,2, Zhao-Xiang Wang*3,4, Yuan-Hsin Lo5, Chaw-Ning Lee3,6, Yun Chang7, Rita Yuchiao Chang7, Chien-Chun Huang3, Tak-Wah Wong3,8,9 1Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, 2Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 3Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 4Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 5Department of Dermatology, Fu Jen Catholic University Hospital, Fu Jen Catholic University, 6Institute of Clinical Pharmacy and Pharmaceutical Sciences, College of Medicine, National Cheng Kung University, 7School of Medicine, National Cheng Kung University, 8Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 9Center of Applied Nanomedicine, National Cheng Kung University The growing incidence of drug-resistant Candida albicans is a serious health issue worldwide. Antimicrobial photodynamic therapy (aPDT) may offer a strategy to fight against drug-resistant fungal infections. The present protocol describes Rose bengal-mediated aPDT efficacy on a multidrug-resistant C. albicans strain in vitro. Behavior Computerized Adaptive Testing System of Functional Assessment of Stroke Gong-Hong Lin1, Yi-Jing Huang1, Yeh-Tai Chou2, Hsin-Yu Chiang3, Ching-Lin Hsieh1,4,5 1School of Occupational Therapy, College of Medicine, National Taiwan University, 2Research Center for Psychological and Educational Testing, National Taiwan Normal University, 3Department of Occupational Therapy, College of Medicine, Fu Jen Catholic University, 4Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital, 5Department of Occupational Therapy, College of Medical and Health Science, Asia University Here, we present a protocol to develop the computerized adaptive testing system of the functional assessment of stroke (CAT-FAS). The CAT-FAS can simultaneously assess four functions (two motor functions [upper and lower extremities], postural control, and basic activities of daily living) with sufficient reliability and administrative efficiency. Bioengineering Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli Tzu-Yuan Chou1, Yung-Shin Sun2, Hsien-San Hou3, Shang-Ying Wu1, Yun Zhu1, Ji-Yen Cheng3, Kai-Yin Lo1 1Department of Agricultural Chemistry, National Taiwan University, 2Department of Physics, Fu-Jen Catholic University, 3Research Center for Applied Sciences, Academia Sinica Micro-fabricated devices integrated with fluidic components provide an in vitro platform for cell studies mimicking the in vivo micro-environment. We developed polymethylmethacrylate-based microfluidic chips for studying cellular responses under single or coexisting chemical/electrical/shear stress stimuli. Behavior Conscious and Non-conscious Representations of Emotional Faces in Asperger's Syndrome Vincent S. C. Chien1,2, Arthur C. Tsai1, Han Hsuan Yang3, Yi-Li Tseng4, Alexander N. Savostyanov5,6, Michelle Liou1,7 1Institute of Statistical Science, Academia Sinica, 2Max Planck Institute for Human Cognitive and Brain Sciences, 3Department of Psychology, Fo Guang University, 4Department of Electrical Engineering, Fu Jen Catholic University, 5State Research Institute of Physiology and Basic Medicine, 6Novosibirsk State University, 7Imaging Research Center, Taipei Medical University An EEG experimental protocol is designed to clarify the interplay between conscious and non-conscious representations of emotional faces in patients with Asperger's syndrome. The technique suggests that patients with Asperger's syndrome have deficits in non-conscious representation of emotional faces, but have comparable performance in conscious representation with healthy controls.
