Chang Gung University College of Medicine 3 articles published in JoVE Medicine Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium Kuo Hsuan Hung1,2,3, Lung Kun Yeh1,2 1Department of Ophthalmology, Chang-Gung Memorial Hospital, 2Chang-Gung University College of Medicine, 3Institute of Clinical Medicine, National Yang Ming Chiao Tung University Here, animal models based on mouse and rabbit are developed for mechanical and chemical injury of corneal epithelium to screen new therapeutics and the underlying mechanism. Immunology and Infection Murine Full-thickness Skin Transplantation Chih-Hsien Cheng*1,2, Chen-Fang Lee*1,2, Madeline Fryer*3, Georg J. Furtmüller3, Byoungchol Oh3, Jonathan D. Powell1, Gerald Brandacher3 1Sidney-Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 2Department of Liver and Transplantation Surgery, Chang-Gung Transplantation Institute, Chang-Gung Memorial Hospital, Chang-Gung University College of Medicine, 3Vascularized Composite Allotransplantation Laboratory, Department of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant. Immunology and Infection In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System Shiun Chang1, Li-Mei Pai2,3, Chien-Kuo Lee1 1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Biochemistry, Chang Gung University College of Medicine, 3Molecular Medicine Research Center, Chang Gung University College of Medicine In this study we present an in vitro culture system that can efficiently generate pDCs by co-culturing common lymphoid progenitors with AC-6 feeder cells in the presence of Flt3 ligand.
