Chang Gung Memorial Hospital and Chang Gung University 1 article published in JoVE Cancer Research Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres Chun-Chia Cheng1, Po-Jui Hsu4, Zong-Lin Sie1, Fang-Hsin Chen1,2,3 1Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences, Chang Gung University, 3Department of Radiation Oncology, Chang Gung Memorial Hospital, 4Department of Laboratory Medicine, Mackay Memorial Hospital Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells.
