The Heart Research Institute

3 articles published in JoVE

• Bioengineering

  
  Molecular Spring Constant Analysis by Biomembrane Force Probe Spectroscopy Peyman Obeidy¹, Haoqing Wang^1,2,3, Mingqin Du¹, Huiqian Hu^1,4, Fang Zhou¹, Haoruo Zhou⁵, Hao Huang⁵, Yunduo Charles Zhao^1,2, Lining Arnold Ju^{1,2,3 1}School of Biomedical Engineering, Faculty of Engineering, The University of Sydney, ²Charles Perkins Centre, The University of Sydney, ³Heart Research Institute, ⁴Department of Chemistry, The Hong Kong University of Science and Technology, ⁵School of Aerospace, Mechanical and Mechatronic Engineering, Faculty of Engineering, The University of Sydney A biomembrane force probe (BFP) is an in situ dynamic force spectroscopy (DFS) technique. BFP can be used to measure the spring constant of molecular interactions on living cells. This protocol presents spring constant analysis for molecular bonds detected by BFP.

• Immunology and Infection

  
  In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages Yunjia Zhang^1,2, Benjamin S. Rayner^1,2, Mathias Jensen³, Clare L. Hawkins^{1,2,3 1}Heart Research Institute, ²Sydney Medical School, University of Sydney, ³Department of Biomedical Sciences, University of Copenhagen Presented here is a protocol to detect macrophage extracellular trap (MET) production in live cell culture using microscopy and fluorescence staining. This protocol can be further extended to examine specific MET protein markers by immunofluorescence staining.

• Medicine

  
  Murine Model of Wound Healing Louise Dunn^1,2, Hamish C. G Prosser^1,2, Joanne T. M. Tan^1,2, Laura Z. Vanags^1,2, Martin K. C. Ng^1,2,3, Christina A. Bursill^{1,2 1}The Heart Research Institute, ²Sydney Medical School, University of Sydney, ³Cardiology Department, Royal Prince Alfred Hospital A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.