Monash Biomedicine Discovery Institute 1 article published in JoVE Biochemistry Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain Mayra A. Machuca1,2, Anna Roujeinikova1,2,3 1Infection and Immunity Program, Monash Biomedicine Discovery Institute, 2Department of Microbiology, Monash University, 3Department of Biochemistry and Molecular Biology, Monash University A procedure is presented for the refolding of the dCACHE periplasmic ligand binding domain of Campylobacter jejuni chemoreceptor Tlp3 from inclusion bodies and the purification to yield milligram quantities of protein.
