University of New South Wales Australia 1 article published in JoVE Medicine Collection, Expansion, and Differentiation of Primary Human Nasal Epithelial Cell Models for Quantification of Cilia Beat Frequency Katelin M. Allan1,2, Sharon L. Wong1,2, Laura K. Fawcett1,2,3, Alexander Capraro1,2, Adam Jaffe1,2,3, Cristan Herbert4, Elvis Pandzic5, Shafagh A. Waters1,2,3 1School of Women's and Children's Health, Faculty of Medicine and Health, University of New South Wales, 2Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health, University of New South Wales, 3 This protocol describes nasal epithelial cell collection, expansion, and differentiation to organotypic airway epithelial cell models and quantification of cilia beat frequency via live-cell imaging and custom-built scripts.
