The Children’s Hospital at Westmead 1 article published in JoVE Biology Anesthesia and Intubation of Preadolescent Mouse Pups for Cardiothoracic Surgery Jianxin Wu*1, Amy M. Nicks1,2, Justin J. Skowno3,4, Michael P. Feneley1,2, Robert M. Graham1,2, Siiri E. Iismaa*1,2 1Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, 2St Vincent’s Clinical School, University of New South Wales Sydney, 3Department of Anaesthesia, The Children’s Hospital at Westmead, 4School of Child and Adolescent Health, University of Sydney Cardiothoracic surgical models in mice >7 days old require intubation, but this is challenging for preadolescent (8-14-day-old) mouse pups and there is little information on anesthetic regimens for intubation. Here, we present dosage regimens of ketamine/xylazine/atropine in 10-day-old C57BL/6J mouse pups that allow endotracheal intubation, while minimizing animal mortality.
