3 articles published in JoVE
Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT Blake J. Cochran1, William J. Ryder2,3,4,5, Arvind Parmar6, Kerstin Klaeser4,5, Anthonin Reilhac7, Georgios I. Angelis4,5, Steven R. Meikle4,5, Philip J. Barter1,5, Kerry-Anne Rye1,5 1School of Medical Sciences, Faculty of Medicine, UNSW Australia, 2Department of Nuclear Medicine, Concord Hospital, 3National Imaging Facility, University of Sydney, 4Brain and Mind Centre, University of Sydney, 5Faculty of Health Sciences, University of Sydney, 6Life Sciences, ANSTO, 7CERMEP This study describes a protocol that uses 18F-FDG and positron emission tomography/computed tomography (PET/CT) imaging, together with kinetic modelling, to quantify the in vivo, real-time uptake of 18F-FDG into tissues.
A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy Victoria C Cogger*1,2,3, Jennifer N O'Reilly*1,2, Alessandra Warren1,2,3, David G Le Couteur1,2,3 1Centre for Education and Research on Ageing & ANZAC Research Institute, University of Sydney and Concord Hospital, 2Ageing and Alzheimers Institute, Concord Hospital, 3Charles Perkins Centre, University of Sydney The fenestrated liver sinusoidal endothelial cell is a biologically important filter system that is highly influenced by various diseases, toxins, and physiological states. These changes significantly impact on liver function. We describe methods for the standardisation of the measurement of the size and number of fenestrations in these cells.
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing Jerry Zhou1, Larissa Belov1, Michael J. Solomon2, Charles Chan3, Stephen J. Clarke4, Richard I. Christopherson1 1School of Molecular Bioscience, University of Sydney, 2Department of Surgery, Royal Prince Alfred Hospital, 3Department of Anatomical Pathology, Department of Anatomical Pathology, 4Department of Medicine, Concord Repatriation General Hospital We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.