3 articles published in JoVE
Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry Sarah E. Ashley1,2, Braydon A. Meyer2,3, Justine A. Ellis2,3,4, David J. Martino2,3,5 1Molecular Genetics of Chronic Inflammation and Allergic Disease, Max-Delbrück Center for Molecular Medicine, 2 Identification of genetic variants contributing to complex human disease allows us to identify novel mechanisms. Here, we demonstrate a multiplex genotyping approach to candidate genes or gene pathway analysis that maximizes the coverage at low cost and is amenable to cohort-based studies.
Measuring Oral Fatty Acid Thresholds, Fat Perception, Fatty Food Liking, and Papillae Density in Humans Rivkeh Y. Haryono1, Madeline A. Sprajcer1, Russell S. J. Keast1 1School of Exercise and Nutrition Sciences, Deakin University Oral chemoreception of fatty acids and the association with diet and fatty food preferences may enable the identification of mechanisms involved with the development of obesity and why dietary changes may be difficult for many individuals.
Metabolic Profile Analysis of Zebrafish Embryos Yann Gibert1, Sean L. McGee1, Alister C. Ward1 1Metabolic Research Unit & Molecular & Medical Research SRC, Geelong, Australia, School of Medicine, Deakin University Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.