Olivia Newton-John Cancer Research Institute 2 articles published in JoVE Biology Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy Adam C. Parslow1,2, Andrew H.A. Clayton3, Peter Lock4, Andrew M. Scott1,2,5,6,7 1Tumour Targeting Laboratory, Olivia Newton-John Cancer Research Institute, 2School of Cancer Medicine, La Trobe University, 3Centre for Micro-Photonics, Faculty of Science, Engineering and Technology, Swinburne University of Technology, 4LIMS Bioimaging Facility, La Trobe Institute for Molecular Science, La Trobe University, 5Department of Medical Oncology, Olivia Newton-John Cancer and Wellnes Centre, Austin Health, 6Department of Medicine, University of Melbourne, 7Department of Molecular Imaging and Therapy, Austin Health Antibodies that bind to target receptors on the cell surface can confer conformation and clustering alterations. These dynamic changes have implications for characterizing drug development in target cells. This protocol utilizes confocal microscopy and image correlation spectroscopy through ImageJ/FIJI to quantify the extent of receptor clustering on the cell surface. Medicine Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice Matthias Ernst1,2,3, Adele Preaudet1, Tracy Putoczki1,2 1The Walter and Eliza Hall Institute for Medical Research, 2The Department of Medical Biology, University of Melbourne, 3Olivia Newton-John Cancer Research Institute We describe methods for longitudinal monitoring of the efficacy of therapeutics for the treatment of colonic pathologies in mice using a rigid endoscope. This protocol can be readily used for the characterization of the therapeutic response of an individual tumor in live mice and also for monitoring potential disease relapse.
